anti ps1  (Proteintech)

Journal: Neural Regeneration Research

Article Title: MicroRNA-502-3p regulates GABAergic synapse function in hippocampal neurons

doi: 10.4103/NRR.NRR-D-23-01064

Figure Lengend Snippet: Summary of antibody dilutions and conditions used in western blot analysis

Article Snippet: PSN1 rabbit polyclonal , 1:1000 , 16163-1-AP , Proteintech, Rosemont, IL, USA.

Techniques: Western Blot

Journal: Neural Regeneration Research

Article Title: MicroRNA-502-3p regulates GABAergic synapse function in hippocampal neurons

doi: 10.4103/NRR.NRR-D-23-01064

Figure Lengend Snippet: Modulation of AD proteins by miR-502-3p. (A) Immunoblot analysis of amyloid precursor protein (APP), tubulin associated unit (Tau), presenilin-1 (PSN1) and presenilin-2 (PSN2) and -actin protein in hippocampal neurons (HT22) cells transfected with miR-502-3p agomiRs and miR-502-3p antagomiRs. The levels of AD proteins were changed in miR-502-3p agomiR and antagomiR transfected cells. (B) Densitometry analysis of APP, Tau, PSN1 and PSN2 blot shows increased protein levels in miR-502-3p agomiR transfected cells compared with scramble control, while the cells treated with miR-502-3p antagomiR shows a reduced level of all AD proteins compared with agomiRs. For statistical analysis, Student’s t -test was used for analyzing the data between three groups of samples such as scramble control, agomiRs, and antagomiRs. Experiments were performed in triplicates. The P -values < 0.05 were considered statistically significant. AD: Alzheimer’s disease; miR: microRNA.

Article Snippet: PSN1 rabbit polyclonal , 1:1000 , 16163-1-AP , Proteintech, Rosemont, IL, USA.

Techniques: Western Blot, Transfection, Control